Oral Sessions (Day1, Day2, Day3)
Poster Presentations
(Day1, Day2, Day3)
Poster Presentations
- Day 2, May 16（Thu.） Room P (Multi-purpose Hall)
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2P-25 PDF
Development of Method for Quantifying Antibody-Drug Conjugates Using LC-MS/MS ‒From Characteristics Analysis to Bioanalysis‒
An analytical method for quantifying ADC in serum with LC-MS/MS has been developed using Kadcyla which is commercially available ADC. As a result of measuring an enzymatic digest of Kadcyla using trypsin, V8 and Asp-N using Q-TOF/MS, 32 of 44 sites of lysine in Kadcyla were identified as conjugation sites of DM-1. The conjugation ratio of DM-1 for each lysine was estimated from the peak area ratio of drug conjugate to non-conjugate. Based on these results, a specific payload-linked peptide was selected from the tryptic digest. By using these peptides, a bioanalytical method with LC-MS/MS was constructed.