Abstract

Oral Sessions

Day 2, May 16（Thu.）　10:50-11:10　Room E (201)

2E-O1-1050　　PDF

Structure Determination of a Novel Phospholipase A2 from the Venom of the Scorpion Liocheles australasiae

(Kyoto Univ.)
^oNaoya Mitani, Mao Yakio, Atsushi Kitanaka, Masahiro Miyashita, Yoshiaki Nakagawa, Hisashi Miyagawa

Scorpions use venom for prey capture and self-defense. The venom contains many bioactive peptides and proteins. In this study, we isolated a Phospholipase A2, named LaPLA2, was isolated from the venom of the scorpion Liocheles australasiae by RP-HPLC and determined its mature structure by enzymatic digestion and mass spectrometric analysis. As a result, we revealed that LaPLA2 is composed of two subunits, the large one with 101 residues and the small one with 9 residues. We also found that LaPLA2 contains an N-glycan moiety, whose structure was estimated by MS/MS analysis.