Abstract

Oral Sessions

Day 2, May 16（Thu.）　10:50-11:10　Room B (102)

2B-O1-1050　　PDF

A Novel Strategy for the Pathological Study of Alzheimer's Disease (AD) Brain with MALDI Imaging Mass Spectrometry in Combination with Shotgun Proteomics

(¹Doshisha Univ., ²Bruker, ³TMIG, ⁴Doshisha Univ.)
^oMasaya Ikegawa¹, Nobuto Kakuda¹, Tomohiro Miyasaka¹, Takashi Nirasawa², Ryo Kajita², Shigeo Murayama³, Yasuo Ihara⁴

Neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of Amyloid β (Aβ) peptides into extracellular plaques of the brain. The Aβ peptides, composed of forty amino acids, are generated from amyloid precursor proteins (APP) by β- and γ-secretases. Aβ is deposited not only in cerebral parenchyma but also in leptomeningeal and cerebral vessel walls, known as cerebral amyloid angiopathy (CAA). While a variety of Aβ peptides were identified, detailed production and distribution of individual Aβ peptides in pathological tissues of AD and CAA is not fully addressed. Here, we develop a novel protocol of MALDI-imaging mass spectrometry (MALDI-IMS) in combination with shotgun proteomics on human autopsy brain tissues to obtain a comprehensive protein mapping.