Abstract

Poster Presentations

Day 1, May 15（Wed.）　　Room P (Multi-purpose Hall)

1P-23　　PDF

Real-Time Analysis and Rapid Screening of Biological Samples Using Remote Sampling Electrospray Ionization Mass Spectrometry

(¹Univ. Yamanashi, ²Univ. Yamanashi, ³Univ. Hops. Leicester, ⁴Nat. Taiwan Univ. Hosp.)
^oKentaro Yoshimura¹, Yuki Yamada², Satoshi Ninomiya², Wen Yuan Chung³, Yu-ting Chang⁴, Ashley Robert Dennison³, Kenzo Hiraoka², Sen Takeda¹, Lee Chuin Chen²

Real-time and in-situ mass-spectrometry analyses of living animal and biological sample were performed using a novel remote sampling electrospray ionization (RS-ESI) probe. Unlike conventional ESI, in which injection or syringe loading is required for sample introduction, the RS-ESI probe ionizes the samples when the sampling capillary is in contact with the sample. As the sampling capillary is electrically held at ground potential, the safety of the animal and operator is assured. The liquid sample is aspirated to the ESI emitter at the other end of the capillary by the Venturi effect. Subsequently, the electrospray is generated when a high voltage is applied to the counter electrode placed inside the ion source chamber. The probe unit is attached to the mass spectrometer with a long flexible tube and its position can be freely manipulated during the analysis. In this report, we demonstrate a real-time analysis of a living mouse liver and an automatic analysis of 138 serum samples using this new technique.