- Day 1, May 15（Wed.） Room P (Multi-purpose Hall)
High Mass Accuracy and Resolution Assured by the Novel Combination of DIUTHAME-IMS/FT-ICR in the Laser Desorption/Ionization Imaging Mass Spectrometry
Recently, novel matrix-free ionization-assisting substrates have been developed based on porous alumina membranes named DIUTHAME (Desorption Ionization Using Through-Hole Alumina MEmbrane). Successful evaluation experiments have been conducted on the feasibility of that membrane for the ionization method using liquid samples. In our current study, we aimed at extending its potential application as an alternative to the matrix to see the molecular distribution of animal tissue through a novel combination of DIUTHAME-IMS and Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. This combination has great advantages on the mass accuracy and resolution. Adult mice brain sections mounted on indium tin oxide (ITO) glass slides were coated with matrix or DIUTHAME, and subsequently measured by SolariX XR (FT-ICR) mass spectrometer in positive ion mode. DIUTHAME-IMS were capable of detecting almost the same molecular species as a matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) with high reproducibility and drastically less background noise. In most cases, the ion images obtained by DIUTHAME-IMS showed better spatial resolution compared to that of the MALDI-IMS. Our current findings suggest that DIUTHAME might be used in place of the matrix and the novel combination of DIUTHAME-IMS/FT-ICR conserves high mass accuracy and resolution in IMS of animal tissue.