Oral Sessions (Day1, Day2, Day3)
Poster Presentations
(Day1, Day2, Day3)
Oral Sessions
- Day 1, May 15(Wed.) 16:25-16:45 Room C (101)
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1C-O1-1625 PDF
Characterization of Induced Pluripotent Stem Cells (iPSCs) and Their Derived Cells by LC/MS/MS
Induced pluripotent stem cells (iPSCs) and iPSC-derived cells are useful in regenerative medicine, disease modeling, and drug safety research. However, directed differentiation to target cell types is still challenging, and in-depth characterizations of iPSCs and iPSC-derived cells are required for commercial application. We have conducted LC/MS-based proteomics, phosphoproteomics, and glycoproteomics for elucidation of differentiation mechanisms and characterization of iPSCs and iPSC-derived cells. Using LC/MS/MS, we simultaneously confirmed up-regulations of multiple proteins and pathways which are related to the differentiated cell functions. Moreover, differentiation stage-specific alteration of glycosylation was observed in several glycoproteins. In this study, we revealed LC/MS/MS is a powerful tool for characterization of iPSC-derived cells and elucidation of their differentiation mechanisms.