Oral Sessions (Day1, Day2, Day3)
Poster Presentations
(Day1, Day2, Day3)
Oral Sessions
- Day 1, May 15（Wed.） 15:45-16:05 Room C (101)
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1C-O1-1545 PDF
A Method for Quantifying Antibody-Drug Conjugates Using LC-MS/MS ‒From Screening Target Peptides to Developing Method for Quantification‒
An analytical method for quantifying ADC in serum with LC-MS/MS has been developed using Kadcyla which is commercially available ADC. As a result of measuring an enzymatic digest of Kadcyla using trypsin, V8 and Asp-N using Q-TOF/MS, 32 of 44 sites of lysine in Kadcyla were identified as conjugation sites of DM-1. The conjugation ratio of DM-1 for each lysine was estimated from the peak area ratio of drug conjugate to non-conjugate. Based on these results, a specific payload-linked peptide was selected from the tryptic digest. By using these peptides, a bioanalytical method with LC-MS/MS was constructed.