日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 1, May 15(Wed.) 15:25-15:45 Room B (102)

Multi-Lipidomics Approaches for Investigating Lipid Metabolism Comprehensively and Unbiasedly

(1RIKEN IMS, 2AMED-PRIME, 3Keio Univ.)
oKazutaka Ikeda1,2, Makoto Arita1,3

Lipid metabolites consist of a wide variety of fatty acids or of polar groups, and some of them are quantitatively and qualitatively variable or oxidizable depending on the situation. To investigate lipid metabolic changes in detail, lipidomics analysis by LC-MS has been wildly applied. However, it remains insufficient to measure them comprehensively and difficult to discover the important metabolic change unbiasedly by conventional approach.
To solve these problems, we present a multi-lipidomics approach which combine non-targeted method using LC/Q-TOF MS and Lipidiscovery (in-house screening software), and targeted method using LC/TQ MS in accordance with lipid properties and the concentration range.
Our advanced lipidomics platform has a strong potential that help to understand the lipid metabolism comprehensively or to identify unknown lipid species in non-biased manners.