プログラム

ポスター発表

第4日　5月18日（金）　　ポスター会場

4P-25　　PDF

Comprehensive workflow for deep and better understanding of peptidome data

(¹新大BBC・ ²新潟大学農学部)
^oShalaby, Amr^1,2・ Hirao, Yoshitoshi¹・ Xu, Bo¹・ Saito, Suguru¹・ Quadery, Ali¹・ Yamamoto, Keiko¹・ Mitsui, Toshiaki²・ Yamamoto, Tadashi¹

In this study, analysis of 24 healthy urine samples resulted in the identification of 432 highly confident native peptides after filtration. Using Long peptide variant (LPV) algorithm generated 229 LPV peptides which considered as precursor native peptides. More investigation of 229 precursor peptides showed that precursor peptides fall into two main categories; (1) Non-truncated precursor peptides category (NTP) which contains 166 precursor peptides without any truncation at N or C terminus and (2) truncated precursor peptides category (TP) which contains 63 precursor peptides truncated to 217 truncated forms at N or C terminus. Prediction of surface accessibility for octapeptide cleavage site (CS) sequence which flanking precursor native peptides (in the context of precursor proteins) showed that 230 of CS are buried and not accessible to peptidases to cleave whereas 227 CS are accessible to peptidases. Prediction of peptidases involved in cleavage of CS flanking precursor native peptides showed that that M10A peptidases family is the most presented family involved in urinary peptide generation. In this study, 13 members of 29 M10A family members were predicted to cleave at 62 distinct cleavage sites with high-predicted contribution of MMP-9 and MMP12.