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ポスター発表

第4日　5月18日（金）　　ポスター会場

4P-21　　PDF

Protein profiling of osteosarcoma tissue and soft callus reveals activation of the unfolded protein response

(¹OLARN center, CMU・ ²CMU・ ³CGI・ ⁴CRI・ ⁵ORTC, CMU)
^oChaiyawat, Parunya¹・ Sungngam, Patsadakorn¹・ Teeyakasem, Pimpisa¹・ Klangjorhor, Jeerawan¹・ Settakorn, Jongkolnee²・ Srisomsap, Chantragan^3,4・ Svasti, Jisnuson^3,4・ Pruksakorn, Dumnoensun^1,5

Survival rate of osteosarcoma patients has not been improved for the past decades due to complexity in genomic background of the disease in which limits a discovery of new therapeutic option. High rate of somatic mutations causes dramatic chaos in various pathways that is potentially revealed at the protein level. This study explores molecular networks associating with oncogenesis of osteosarcoma using proteomics and bioinformatics tools. Since clinical specimens are the most relevant disease-related source, expression patterns of proteins in osteosarcoma tissues were compared to soft tissue callus from donors. Soft callus was used as a non-cancerous control tissue for the first time, since high density of osteoblasts were observed by histological evaluation. Using two-dimensional electrophoresis and LC-MS/MS, we identified 33 differentially expressed proteins. Functionally grouped network of the overexpressed proteins created by ClueGo and CluePedia application demonstrated that the unfolded protein response (UPR) pathway was activated mainly through ATF6 arm in osteosarcoma. Targeting the UPR is very promising for the treatment of various cancers that warrant the use of FDA-approved drugs and chemical agents. Therefore, this finding suggests a novel target for the treatment of osteosarcoma with available therapeutic agents that accelerates an improvement in osteosarcoma patients' clinical outcomes.