ポスター発表
- 第4日 5月18日（金） ポスター会場
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4P-18 PDF
血漿エクソソームを対象とした新規大腸癌バイオマーカー探索
The recent development of mass-spectrometry-based technologies has been providing unprecedented insights in biomarker discovery. However, it is still regarded as extremely challenging to provide high-reproducibility in plasma proteome analysis for a small amount of protein. Isolating extracellular vesicles (EVs) from plasma is one of ideal methods to overcome this problem in the point of reducing abundant proteins. We attempted to gradually narrow down biomarker candidates (BCs). We isolated EVs from plasma in colorectal cancer (CRC) patients (n=59) and healthy controls (n=59) by using ultracentrifugation. LC-MS/MS was used to provide an overview of plasma EVs protein profile (Dataset 1). Primary BCs were selected by three datasets: PubMed literature search for proteins that had been reported to be functionally correlated to the pathogenesis of CRC (Dataset 2); ExoCarta database research for proteins that had been reported their expression in EVs (Dataset 3). LC-SRM/MS was used to obtain absolute quantification of primary BCs for selection of secondary BCs. MS-based Quantification By isotope-labeled Cell-free products (MS-QBiC) 1) provided high-throughput preparation of internal standards. As validation phase, we used isolated EVs from plasma in CRC patients (n=50) and healthy controls (n=50) to evaluate the performance of secondary BCs. Eventually, novel biomarker were identified.