プログラム

ポスター発表

第4日　5月18日（金）　　ポスター会場

4P-17　　PDF

慢性腎臓病の腎生検組織のプロテオーム解析

(¹新大BBC・ ²新潟病院・ ³信楽園病院)
^o山本恵子¹・ 平尾嘉利¹・ 藤中秀彦²・ 宮崎滋³・ 許波¹・ Elguoshy, Amr¹・ 山本格¹

Chronic Kidney Disease (CKD) is a serious disease, which progresses to the end-stage chronic renal failure and patients with CKD may be treated with dialysis therapy or kidney transplantation. By pathology, CKD is classified into several diseases, such as diabetic nephropathy, IgA nephropathy, nephrosclerosis and others. To understand molecular mechanisms of the progression of CKD, especially in the glomerulus as the major injury site is the glomerulus, glomerular sections were microdissected from formalin-fixed paraffin-embedded (FFPE) kidney biopsy tissues of patients with CKD or normal kidney tissues which were removed for cancer treatment and analyzed by a label-free LC-MS quantitation (modified normalized spectral index, SIN). Proteins identified in the glomerulus uniquely in each disease or with more than 2-fold SIN value were analyzed by DAVID pathway analysis. Several unique pathways were elucidated in each CKD disease by the analysis, which may promote development of new radical therapies in the near future.