ポスター発表
- 第4日 5月18日（金） ポスター会場
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4P-13 PDF
Immediate adaptation signalling buffers response to EGFR-TKI treatment
Molecular response profiling at mRNA and protein level in a timecourse experiment after EGFR-TKI treatment indicated massively altered mRNA and protein expression levels already 24 hours after treatment. Our hypothesis here was that some of this regulation potentially helps the cells to escape from the effects of EGFR inhibition.
In order to identify such signaling, we selected 750 genes that were regulated in response to EGFR-TKI treatment and knocked down their expression in a siRNA screen. This screen showed that silencing of ERBB2, BCL2L1 (Bcl-XL), BCL6 and TFEB (all unregulated or activated after EGFR-TKI treatment), resulted in reduced viability and/or increased apoptosis suggesting that they may be involved in EGFR-TKI escape mechanisms. We suggest the four genes as potential candidate targets for EGFR-TKI combination therapy.