オーラルセッション
- 第4日 5月18日（金） 10:40～11:00 C会場（星雲2）
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4C-O1-P-1040 PDF
バイオ医薬品の特性解析：肝細胞増殖因子のジスルフィド解析法開発
Human hepatocyte growth factor (hHGF) is about 90 kDa heterodimer protein containing 41 cysteine residues. The positions of twenty disulfide bridges of the recombinant protein expressed from CHO cells were determined by the combination of unique enzymatic digestion of the protein and the LC/MS/MS analysis. In addition, it was also identified that an unpaired cysteine was highly cysteinylated. The protein is currently under development as a therapeutic protein for liver failure diseases.