オーラルセッション
- 第4日 5月18日（金） 10:45～11:00 B会場（星雲1）
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4B-O1-P-1045 PDF
Soybean Proteomics: A Leading Tool in Exploring Effects of Flooding Stress using Proteomic Technique
Among legumes, soybean ranks high in susceptibility to flooding stress. With this respect and due to high importance of soybean production, soybean must be evaluated against flooding stress. To understand soybean protein networks regulating responses towards flooding stress, gel-based proteomic technique was used. Among various mechanisms adapted by soybean against flooding stress, regulation of carbon metabolism to meet cells energy requirements to cope with the stress is common in all cases. Soybean was affected more severely by flooding stress compared to low oxygen stress, indicating that protein destination/storage and disease/defense related proteins were significantly changed in flooded seedlings leading to more growth suppression. Decreased isoflavone reductase in root, shoot and leaf, and upregulation of its gene at transcript level, clearly indicated that flooding stress interfered at translation level. Inoculation of soybean with Bradyrhizobium japonicum resulted in regulation of disease/defense and metabolism-related proteins causing increased number of root hair during early symbiotic differentiation. Proteomic results also clarified that recovery after flooding may involve alteration of cell structure through changes in cell wall metabolism and cytoskeletal organization. These results suggest that proteomics provide a reliable method to unravel the regulation of complex protein network involved in soybean response towards flooding stress.