プログラム

オーラルセッション

第4日　5月18日（金）　09:15～09:30　B会場（星雲1）

4B-O1-P-0915（2P-57）　　PDF

微量組織を対象としたペプチドーム解析法の確立と応用

(¹北里大理・ ²北里大理プロテオミクスセンター・ ³北里大医)
^o中川譲¹・ 山田拓也¹・ 角田貴樹¹・ 藤本和実^1,2・ 板倉誠³・ 小寺義男^1,2

The low-molecular-weight protein and peptide component of tissue includes members of several physiologically important classes, such as cytokines, chemokines, peptide hormones, and neuropeptide. However, method for comparative analysis of peptides intended for a small amount of sample is required to discover biomarker. In this study, we established a method to analyze peptides in detail from a small amount of tissues. The analysis enabled us to identify about 2,500 peptides derived from 921 proteins that include 13 hormone precursor proteins from only 3 mg of mouse brain whole lysate.