プログラム

オーラルセッション

第4日　5月18日（金）　15:25～15:45　A会場（オービットホール）

4A-O2-P-1525　　PDF

酵素活性の網羅的解析（Enzymomics）法による疾患関連タンパク質の探索

(¹東大院薬・ ²東大院医・ ³東大創薬機構・ ⁴sainome・ ⁵AMED-CREST)
^o小松徹¹・ 小名木淳¹・ 市橋裕樹¹・ 柳光一¹・ 荻原洲介¹・ 河村眞紀子⁴・ 下田昌弘⁴・ 長野哲雄³・ 浦野泰照^2,5

We would like to introduce the study of enzymes using the small molecular fluorescent substrate probes that can report enzymes' live functions in bio-samples. We have developed the methodology of characterizing disease-related enzymes by globally monitoring their activities using the library of substrates, and discovering and characterizing ones that showed alternation in disease-derived samples. In one example, a global monitoring of the peptide-metabolizing enzymatic activities led to the discovery of novel endopeptidase activity that was elevated in colorectal tumor cells. We expect that the omics of enzyme, enzymomics, will proceed for further characterization of disease-related proteins as potential drug targets or biomarkers.