オーラルセッション
- 第4日 5月18日（金） 13:00～13:10 A会場（オービットホール）
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4A-L-P-1300 PDF
小児のピロリ菌慢性感染を予防するためのCagA蛋白抗原ペプチドを用いた測定法の開発
Chronic infection with East-Asian strains of pathogenic bacteria of Helicobacter pylori (Hp) is a risk factor for not only atrophic gastritis causing gastric cancer in adult but also idiopathic thrombocytopenic purpura or iron deficient anemia in children. An ELISA assay with protein antigenic epitopes of cytotoxin associated gene A (CagA) can be a powerful tool for the diagnosis of Hp infection to prevent chronic infection. Those epitopes were identified by catalogue antigenic proteins of a Japanese strain CPY2052 by two-dimensional electrophoresis followed by immunoblot and LC-MS/MS analysis. Each of the major epitopes of RDLEDKLWAKGLSpQEANK and QKITDKVDNLNQAVSETKL determined by peptide/protein-combined array chip method was found to contain amino acid residues in domain II and III unique to the East-Asian strains of Hp, respectively. (PLoS One. 2014 9(8) e104611) We developed a new ELISA assay using synthetic peptides mimicry of those major epitopes recognized by antibodies in sera and examined the availability of ELIA assay with sear from 225 children of 0-12 y-o in the comparison with a commercially available method of H. pylori-Latex SEIKEN (No. 22800EZX00031000). The rate of infection with Hp was also estimated by H. pylori-Latex SEIKEN with sera from 890 children living in Shunan area to be 1.24%.