プログラム

ポスター発表

第3日　5月17日（木）　　ポスター会場

3P-39　　PDF

High-Definition Mass Spectrometryによるヒト肝がん組織のメタボローム解析

(¹東北大メガバンク・ ²東北大院医・ ³AMED-LEAP・ ⁴東北大院薬・ ⁵東大院医・ ⁶東大病院)
^o三枝大輔^1,2,3・ 永井滉士⁴・ Baasanjav, Uranbileg^3,5,6・ 蔵野信^3,5,6・ 長谷川潔⁶・ 國土典宏⁶・ 池田均^3,5,6・ 矢冨裕^3,5,6・ 富岡佳久⁴・ 青木淳賢^3,4

Hepatocellular carcinoma (HCC) accounts for approximately 90% of primary liver cancers and is the fifth most common cancer in the world. It is assumed that the number of HCC cases will continue to increase, and biomarkers that are related to the degree of tumor progression and cellular characteristics and that can provide clinical information linked to treatment are required. Metabolomics has been developed as one of the post-genomic research fields and is a promising avenue for discovery of HCC biomarkers. Especially, the global metabolomics (G-Met) can analyze metabolic pathways and obtain new knowledge, such as the detection of unknown metabolites. However, G-Met has following problems: (1) the comprehensiveness and reproducibility of the measurement method to cover a wide range of metabolites; (2) the identification of compounds; and (3) the acquisition of information relating to the spatial localization of the compounds. Regarding problem; (1) we used a mixed-mode column that is based on a combination of ion exchange and reversed phase technologies; (2), we used high-definition mass spectrometry, which can separate ions by ion mobility; (3) we used desorption electrospray ionization-MS imaging, which incorporates an electrospray probe and LC-MS.