日本質量分析学会 第66回質量分析総合討論会

プログラム

ポスター発表

第3日 5月17日(木)  ポスター会場

光反応による細胞内ミトコンドリア代謝物分析法の開発

(静岡県大薬)
o田中奈津美水野初高山卓大佐々木崇光南哲平豊岡利正吉成浩一轟木堅一郎

Mitochondria are essential organelle for energy production and cellular existence and they regulate many cellular functions. In order to clarify the detailed mechanisms of cell phenomena, accurate analytical methods for mitochondrial metabolites have been demanded. However, the conventional metabolic approaches are difficult to know the localization of cellular metabolites because these methods analyze large amounts of cells or tissues as homogenized samples. In this study, in order to analyze metabolites located in the mitochondria, we developed an analytical method for metabolites in mitochondria by using mitochondrial localized photoaffinity probe. Our synthesized probe has both diazirin moiety as photoaffinity unit and rhodamine B as a mitochondrial affinity unit, respectively. From the result of fluorescence microscope observation, it was confirmed that this probe was localized in mitochondria of HepG2 cells. Thus, the method would be useful for selective analysis of mitochondrial metabolites.