プログラム

ポスター発表

第3日　5月17日（木）　　ポスター会場

3P-34　　PDF

高感度nano-LC/MS/MSを基盤とした1細胞メタボローム分析システムの開発

(九大生医研)
^o中谷航太・ 和泉自泰・ 秦康祐・ 馬場健史

Cell is the structural, functional, and biological minimum unit of all living organisms. Conventional metabolome analysis requires large amount of samples such as 10–15 mg of tissue or 10⁵–10⁶ cells. Therefore, information garnered from the analysis of samples extracted from a specific tissue or a single-cell is expected to advance knowledge of the physiological heterogeneity. The aim of this study is to develop analytical system for single-cell metabolomics. In this study, we developed an analytical system for single-cell hydrophilic metabolites analysis using a combination of single-cell sample preparation technique (i.e., cell isolation, lysis, and injection) and highly sensitive nano-liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). The developed analytical system is a new tool for single-cell metabolome analysis and thus will facilitate future research in tumor heterogeneity.