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ポスター発表

第3日　5月17日（木）　　ポスター会場

3P-10　　PDF

骨格筋タンパク質の比較定量解析手法の確立

(¹横浜市大先端研・ ²横浜市大医学部・ ³岐阜大)
井野洋子¹・ 大平宇志¹・ 森山佳谷乃¹・ 中居佑介¹・ 木村鮎子¹・ 熊谷研²・ 齋藤知行²・ 森田啓之³・ 木村弥生¹・ ^o平野久¹

In this study, we aimed to establish a technique for comparative quantitative analysis of the skeletal muscle proteome. Skeletal muscle extracts from a mouse hind limb were fractionated into soluble and insoluble fractions, and then analyzed by LC-MS/MS to identify the constituent proteins. This analysis identified 505 and 418 proteins in the soluble and insoluble fractions, respectively. A total of 674 non-redundant proteins were identified, approximately 2-fold more than in a non-fractionated sample. Furthermore, we used this technique to analyze a large number of skeletal muscle samples obtained from mice bred under various conditions, and integrated the MS data using quantitative analysis software. Approximately 1,200 skeletal muscle proteins were quantitatively identified with high reproducibility.