日本質量分析学会 第66回質量分析総合討論会

プログラム

ポスター発表

第3日 5月17日(木)  ポスター会場

Evaluation of a peptide selection algorithm for trapped ion mobility with parallel accumulation serial fragmentation (TIMS PASEF) on a Q-TOF instrument timsTOF Pro with PASEF for shotgun proteomics

(1ブルカージャパン2Bruker Daltonik3Max Planck)
o梶田遼1Lubeck, Markus2Kaspar-schoenefeld, Stephanie2Goedecke, Niels2Raether, Oliver2Drechsler, Nicole2Krause, Michael2Koch, Scarlet2Koch, Heiner2Meier, Florian3Cox, Jürgen3Mann, Matthias3

The “Parallel Accumulation Serial Fragmentation” (PASEF) method for trapped ion mobility (TIMS) quadrupole time of flight (QTOF) instruments enables faster acquisition of fragment ion spectra from isolated precursor ions, without sacrificing spectral quality. During an ion mobility scan the quadrupole switches its isolation position several times, isolating a certain ion species only for the periods of time it actually elutes from the mobility separation device as a condensed package. A modified timsTOF instrument (Bruker) connected to a nanoElute UHPLC (Bruker) was used to test this approach for proteomics applications.