ポスター発表
- 第3日 5月17日（木） ポスター会場
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3P-07 PDF
LC/MS/MSを用いた神経分化プロテオミクスによる神経分化関連分子マーカー探索
Induced pluripotent stem cell (iPSC)-derived neurons are expected to become powerful tools for the study of neurological disease mechanisms and drug safety. The purity, maturity and function of cells should be verified with appropriate methods to ensure safe and effective use of cells. However, only a few biomarkers are available for iPSC-derived neurons. Novel neural markers, specific to each stage in neural differentiation, are urgently required. In this study, we used quantitative proteomic approaches to extract proteins that are expressed specifically in iPSCs, iPSC-derived neural stem cells (NSCs), neural progenitor cells (NPCs), and dopaminergic neurons (DAs). The efficacy of proteins as specific biomarkers of differentiation stages was assessed through functional analysis using gene ontology (GO) and pathway analysis. We identified NSC and NPC marker candidates based on their functions and expression timings in neural cells. Furthermore, we revealed that proteomics using nanoLC/MS/MS could identify population of neurons.