ポスター発表
- 第3日 5月17日（木） ポスター会場
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3P-01 PDF
nanoLC-MSを用いたインタクトタンパク質の高感度分析
Protein identification is usually done by “bottom-up” proteomics, in which the proteins are digested by trypsin prior to LC-MS/MS analysis. However, in case of truncated protein for example, clarification of the terminal amino acid position is often difficult by “bottom-up” proteomics. Although using conventional LC-MS allows us to detect truncated proteins efficiently, it is difficult when the amount of truncated proteins is too low. Therefore, it is important to detect intact protein molecules by nanoLC-MS. In this presentation, a monolithic silica capillary column that was developed by Shinwa Chemical Industries Ltd. was connected to nanoLC-MS to evaluate the performance using several proteins.