- 第3日 5月17日（木） 17:15～17:35 A会場（オービットホール）
Proteomics and Metabolomics approaches for discovery of potential candidate biomarkers for Coronary Artery Disease
Coronary artery disease (CAD) is one of the largest causes of death worldwide, yet the traditional risk factors, although useful in identifying people at high risk, lack the desired predictive accuracy. Nutritional intake has been considered as one of the major factor for developing cardiovascular diseases. We have previously shown that vitamin B12 deficiency is associated with the risk of Coronary heart disease in India. Recently we have also shown in a rat model that maternal vitamin B12 deficiency led to dyslipidemia
This study was aimed to identify differentially expressed proteins in stable CAD patients. In plasma proteomics experiment, we found four proteins (Apo A1, ApoA4, Apo C1 and albumin) that could account for approximately 88% of the CAD cases after ROC analysis. Interestingly, all these proteins, involved in the reverse cholesterol pathway were down regulated which suggests that impairment of reverse cholesterol pathway could eventually accelerate the progression of CAD. Further, we used an untargeted LC-MS based metabolomics approach for the identification of metabolic markers in CAD. This led to the identification of several metabolites involved in phospholipid metabolism, monoglyceride metabolism, sphingolipid metabolism etc whose levels were significantly different in CAD cases.