日本質量分析学会 第66回質量分析総合討論会

プログラム

オーラルセッション

第3日 5月17日(木) 10:45~11:00 A会場(オービットホール)

様々な技術融合によるプロテオームの量的および質的解析手法

(明大農)
o紀藤圭治

Proteomic methods using mass spectrometry have been developed by combination of a variety of techniques. Here we introduce our originally developed strategies in combination with different kinds of techniques along with obtained results. Absolute quantification relies on adequate standards. For PCR-based measurement of individual transcripts, genomic DNA can be used as a native standard, an original concatenated standard containing tandem arrays of each transcript sequence. This concept was applied to proteomics to generate peptide-concatenated standard. We are also in progress of construction of genetically modified yeast cells where unique peptide-tag adequate for mass spectrometric detection is introduced into genomic DNA encoding each protein. A strategy using unique tag has been utilized for comprehensive genetic analysis of yeast deletion library. Generation of yeast cells that have unique tags for a multitude of proteins would be feasible by use of CRISPR/Cas9 gene editing method. Asymmetrically segregation of older proteins between mother and daughter cells, possibly related to aging process, can be analyzed by combination with previously established technique for yeast cell biology. Thus, as we all know, development of unique proteomic strategies relies on a variety of classical and latest techniques and consecutively important for advance in proteomic study.