オーラルセッション
- 第3日 5月17日（木） 09:00～09:30 A会場（オービットホール）
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3A-O1-P-0900 PDF
標的プロテオミクスの技術発展と応用
Targeted proteomics is the LC-MS based method, which identifies and quantifies target peptides from chromatograms extracted from MS data, and getting popular and matured not only in proteomics research but also in basic life sciences and drug development. In the early period, targeted proteomics was performed by MS data acquisition with SRM/MRM and selected and Method of the Year 2012 in Nature methods. To date, various technical progresses have been achieved for improving the ability of targeted proteomics. In the acquisition mode, Q-TOF and orbitrap instruments were introduced in targeted proteomics, and HR-MRM and PRM was developed. Data Independent Acquisition (DIA, SWATH-MS) is a breakthrough methodology in targeted proteomics, which enables comprehensive peptide quantification by extracted ion chromatograms. DIA data contains huge MS data, and data processing methodologies and software have been intensively developed. Because the advantage of targeted proteomics is quantitative ability in addition to sensitivity, sample preparation and target peptide selection are also important processes to obtain reliable quantitative data. Now, we can use various set of targeted proteomics system suitable for each research purpose. In this lecture, we will introduce recent technical progresses of targeted proteomics and our latest application to life science and biomarker research.