日本質量分析学会 第66回質量分析総合討論会

プログラム

ポスター発表

第2日 5月16日(水)  ポスター会場

タンパク質の存在量ならびに機能状態を対象とした比較分析法の確立と応用

(1北里大理2北里大医3北里大・理・疾患プロテオミクスセンター)
o紺野亮1平松夏奈1伊藤大晃1樋口雅崇1板倉誠2小寺義男1,3

Recent technological advances in shotgun proteomics have made human proteome catalogues available to the scientific community. However, it is difficult to obtain information of cleavage and interaction of proteins. On the other hand, the method referred to as the GeLC-MS/MS based on electrophoresis is examine functional status of protein such as proteolytic events, protein interaction, and post translational modification. In this study, we developed the method that combine GeLC-MS/MS with stable isotope labeling of proteins prior to electrophoresis. It is possible to comparatively analyze functional status of proteins in detail. Here, we applied this strategy to analyze alternation of protein expression and function in mouse hippocampus induced by water immersion restraint stress.