ポスター発表
- 第2日 5月16日（水） ポスター会場
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2P-35 PDF
細胞内分子イメージングに向けた大気圧レーザーイオン化質量分析のための透過照射光学系の開発
In imaging mass spectrometry (IMS), the cellular scale resolution is needed for development of new drugs and pathological analysis. Although cellular level IMS is practically accomplished with a spatial resolution of 5–10 μm using a commercial instrument, the technology has still challenging problems, and spatial resolution less than 1 μm is required to observe intracellular molecules. Moreover, the biological samples such as cultured cells should be placed in the atmospheric pressure to maintain the alive condition. These problems are originated in the optical system, so this system called reflection geometry which is employed in most of all IMS instruments is not suitable for high spatial resolution IMS. Therefore, we developed the new one called transmission geometry optical system in which an objective lens was placed behind a transparent sample plate at the atmospheric pressure. In this system, it is easy to collect ions because of no obstacles and the optical component which has high numerical aperture can be employed to make small laser spot size. In this study, mass spectra of crystal violet (CV) using atmospheric pressure laser ionization were measured and the optical system was evaluated.