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ポスター発表

第2日　5月16日（水）　　ポスター会場

2P-34　　PDF

Heat stabilizationを利用したラット転移性肝癌の¹³C標識Glucose/Glutamineトレース解析

(¹京大病院・ ²慶大医・ ³同志社大生命医)
^o井口公太¹・ 森野甲子郎¹・ 西野裕人¹・ 杉浦悠毅²・ 池川雅哉³・ 上本伸二¹

Heat Stabilization (HS) allows us to provide nearly in vivo information about metabolites, which are easily susceptible to ex vivo degradation. We previously reported that the appropriate visualization of adenosine triphosphate (ATP) in the liver using imaging mass spectrometry (IMS) could be obtained from HS fresh samples not from HS frozen ones. In this study, comprehensive quantitative approach to the mouse liver revealed that the various metabolites including ATP could be classified according to the presence or absence of HS treatments. In the metastatic liver cancer rat models, the cancer-specific pathway was elucidated by the metabolic flux analyses using ¹³C-labeled glucose/glutamine tracers. We could also visualize the heterogeneity of the metabolic processes on the cancer and non-cancer tissues using IMS.