日本質量分析学会 第66回質量分析総合討論会

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第2日 5月16日(水)  ポスター会場

Comparatively Proteomic Analysis Reveals the Diminished Capacity of Photosynthesis in PPO-expression-silenced Leaves of Clematis terniflora DC.

(Zhejiang Univ.)
Chen, XiHuang, WeiWang, TantanoYang, BingxianTian, Jingkui

As commonly known, polyphenol oxidase (PPO) catalyzes the o-hydroxylation of monophenols and the oxidation of o-diphenols to quinones. However, although the interaction between PPO and photosynthesis has been proposed, little has been done in the living plant tissues. In order to explore the in vivo physiological functions of PPO, especially the potential involvement in photosynthesis, a model with decreased expression and enzymatic activity of PPO was constructed on Clematis terniflora DC. using virus induced gene silencing (VIGS) technology. Proteomics was performed to identify the differentially expressed proteins (DEPs) in model plant (MP) and empty vector plants (EP). The DEPs were mainly involved in translation, chloroplast, chloroplast thylakoid membrane, and cytosolic small ribosomal subunit. Further analysis showed proteins related to ribosome and photosynthesis were decreased with the silencing of PPO expression in MP than that in EP. The abundances of erredoxin-NADP+ reductase, plastoquinol-plastocyanin reductase, and ATP synthase were decreased in MP than that in EP. Moreover, the interference of PPO expression led to a supplementary decrease in oxidative phosphorylation when under a binary stress combined with UV-B and dark. These results indicate that PPO plays key roles in photosynthesis through participating in the processes of electron transport and oxidative phosphorylation.