プログラム

ポスター発表

第2日　5月16日（水）　　ポスター会場

2P-01　　PDF

プロテオミック反応はピスタチオ地下茎の塩分ストレスに対するシグナル伝達と細胞骨格再配置を明らかにする

(¹フロリダ農工大・ ²タブリズ大学・ ³クエイドアザム大学・ ⁴前橋工科大)
Katam, Ramesh¹・ Akbari, Mohammad^1,2・ Mehana, Nasar²・ Mustafa, Ghazala³・ ^o坂田克己⁴・ Johnson, Kala¹

Pistacia vera L., cultivated in arid and semi-arid regions, is one of the most important nuts. The mechanisms underlying salinity tolerance in this plant is poorly understood. Five one-year-old pistachio rootstocks were treated with four saline water regimes for 100 days and physiological, biochemical and proteomic analysis were carried out. Salinity decreased total chlorophyll content and carotenoids in the leaves, and ascorbic acid and total soluble proteins in both leaves and roots. Total phenolic compounds, proline, glycine betaine, and H2O2 content increased in all rootstocks. Three different ion exclusion strategies were observed in rootstocks, (i) Na+ exclusion in UCB-1, (ii) Cl- exclusion in Badami, (iii) and similar concentrations of Na+ and Cl- in the leaves and roots of Ghazvini, Akbari and Kale-Ghouchi.

High throughput comparative proteomics of roots identified 153 more abundant and 69 low abundant proteins in UCB-1 and 340 more abundant and 18 low abundant proteins in Akbari. Majority identified proteins have the functions related to stress responsive proteins, signal transduction, cell wall and cytoskeleton metabolism. The data suggests a strong linkage of molecular mechanism with the physiological traits in the cultivars with various salt tolerances, and lead to further functional elucidation to improve salt tolerance.