日本質量分析学会 第66回質量分析総合討論会

プログラム

オーラルセッション

第2日 5月16日(水) 18:05~18:20 B会場(星雲1)

マススペクトルにおけるペプチド同位体比の精確性

(1京大院薬2京大院工)
o木部航希1吉沢明康1田畑剛1吉井和佳2石濱泰1

Isotope distribution of peptide peaks in mass spectra can be used to support the peptide identification, although it has not been so frequently used in proteomics. In this study, we examined the accuracy of the observed isotope distribution of tryptic peptides. We evaluated several factors to influence the similarity between the observed and theoretical isotope distribution. As a result, it was found that the absolute intensity as well as the charge states were the two major factors to affect the accuracy in measuring the isotope distribution of peptides.