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第2日　5月16日（水）　16:00～16:20　B会場（星雲1）

2B-O2-M-1600　　PDF

MALDI-TOF/TOFを用いる20keV高エネルギーCIDによるペプチド分解の新局面

(¹横市大院生命ナノ・ ²島津最先端研)
^o高山光男¹・ 関谷禎規²・ 岩本慎一²・ 田中耕一²

Positive-ion CID spectra of peptides obtained with 20keV high-energy TOF/TOF MS have been analyzed from the standpoints of selective/enhanced cleavage of Asp/Glu/Cys-Xxx, Xxx-Pro and Gly-Xxx residues by comparing with low-energy CID. The high-energy CID spectrum of a model peptide BSA1-48 containing Asp, Glu, Cys, Pro and Gly residues showed preferential b- and y-ions originated from the cleavage at C-N bond of Asp-Xxx residues, which is a characteristic of low-energy CID. The selective/enhanced cleavage of Asp-Xxx residues in high- and low-energy CID could be explained by assuming the pre-formation of an intramolecular hydrogen-bonding state (Fig. 1left).