オーラルセッション
- 第2日 5月16日(水) 10:25~10:45 B会場(星雲1)
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2B-O1-P-1025 PDF
陸上植物のアブシシン酸応答におけるタンパク質リン酸化ネットワークの解析
Abscisic acid (ABA) and its signaling system are important for land plants to survive in terrestrial conditions. Protein phosphorylation/dephosphorylation has a central role in ABA signaling, mediated by protein phosphatase 2C (PP2C) and SNF1-related protein kinase 2 (SnRK2). Recently, we are taking a phosphoproteomic approach to elucidate the ABA signaling network in multi-species of land plants. For example, Physcomitrella patens is a model species of basal land plants. Our phosphoproteomic analysis detected 4,630 phosphopeptides from P. patens wild-type and two ABA-responsive mutants, such as a PP2C disruptant (ppabi1a/b), and an ARK-defective mutant (AR7), identified as an upstream regulator of SnRK2. Our data revealed: 1) the entire ABA-responsive phosphoproteome in P. patens was quite diverse, 2) P. patens PP2C regulated multiple pathways in addition to the ABA response, and 3) ARK is mainly involved in ABA signaling. Taken together, we propose that the core ABA signaling pathway is essential in all land plants; however, some ABA-responsive phosphosignaling uniquely developed in bryophytes during the evolutionary process.