ポスター発表
- 第1日 5月15日（火） ポスター会場
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1P-50 PDF
出芽酵母の短寿命株と野生株におけるタンパク質不均等分配の比較解析
Budding yeast cells asymmetrically divide into mother and daughter cells. The number of daughter cells produced from mother cell is limited; after production of 20~30 daughter cells, mother cells no longer divide in consequence of aging, called as replicative life span (RLS). In contrast, daughter cells produced from older mother cells are rejuvenated to replicate 20~30 times. Asymmetric inheritance of a variety of aberrant components, including nucleic acids, proteins, and organelle, can have an impact on RLS and aging of mother cells. We have a hypothesis that accumulation of older proteins asymmetrically segregated into mother cells could promote aging process.
We have originally developed a strategy that allows us to identify asymmetrically inherited protein, by combination of classically mother and daughter cell separation procedure, labeling process of newly-synthesized proteins with stable isotope, and quantitative mass spectrometric analysis. In our previous study, more than 20 asymmetrically inherited proteins were successfully identified in wild type strain of Saccharomyces cerevisiae. We would like to show a difference in asymmetrically inherited proteins between wild type strain and short-lived mutant and present their relation to RLS and aging process.