ポスター発表
- 第1日 5月15日(火) ポスター会場
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1P-12 PDF
GC-NCI-MSを用いた糖リン酸の高感度分離分析法の開発とがん細胞株の13C代謝フラックス解析への応用
A method for sugar phosphates analysis was developed using GC-NCI-MS. Baseline separation of sugar phosphate isomers and detection of molecular ion in ten nano-molar range were achieved. The method was applied to the measurement of 13C-labeling of sugar phosphates for 13C-metabolic flux analysis of MCF-7 human breast cancer cell line. Employment of 13C-labeling of sugar phosphates improves the flux estimation of bidirectional reactions in glycolysis and the pentose phosphate pathway.