プログラム

ポスター発表

第1日　5月15日（火）　　ポスター会場

1P-12　　PDF

GC-NCI-MSを用いた糖リン酸の高感度分離分析法の開発とがん細胞株の¹³C代謝フラックス解析への応用

(¹阪大院情報・ ²島津・ ³阪大院工)
^o岡橋伸幸¹・ 前田昂亮¹・ 川名修一²・ 飯田順子^2,3・ 清水浩¹・ 松田史生¹

A method for sugar phosphates analysis was developed using GC-NCI-MS. Baseline separation of sugar phosphate isomers and detection of molecular ion in ten nano-molar range were achieved. The method was applied to the measurement of ¹³C-labeling of sugar phosphates for ¹³C-metabolic flux analysis of MCF-7 human breast cancer cell line. Employment of ¹³C-labeling of sugar phosphates improves the flux estimation of bidirectional reactions in glycolysis and the pentose phosphate pathway.