プログラム

ポスター発表

第1日　5月15日（火）　　ポスター会場

1P-04　　PDF

血清エクソソームのプロテオミクスによる新規COPDバイオマーカー同定

(¹阪大院医・ ²医薬基盤健栄研)
^o木庭太郎¹・ 武田吉人¹・ 玄山宗到¹・ 滝本宣之¹・ 松木隆典¹・ 木田博¹・ 白水崇²・ 鳴海良平²・ 朝長毅²・ 熊ノ郷淳¹

Introduction: There are no specific biomarkers (BM) for chronic obstructive pulmonary disease (COPD). In order to get novel BM for COPD, we focused on proteins of serum exosomes and performed a quantitative high throughput proteomics using isobaric tags, tandem mass tag system technique (TMT)-based analysis; and subsequent target proteomics, multiple reaction monitoring (MRM).
Methods: Ten COPD patients, and six healthy controls were included in this study. Serum exosomes were isolated by ultracentrifugation. A TMT-based quantitative proteomic analysis and subsequent MRM verification were performed. In addition, bioinformatics technology was applied to analyze the exosomal proteins using ingenuity pathway analysis (IPA).
Results: Isolated exosomes were confirmed by transmission electron microscope, immunoblot, and the qNano system. From TMT proteomics, we obtained 600 proteins. Among them, 34 proteins were upregulated in the COPD patients compared with healthy controls. Of note, serum exosomes contained abundant membrane proteins as well as extracellular matrix proteins. Moreover, exosomal proteins from COPD patients reflected its disease signature such as inflammatory response and metabolic disease by IPA. Among 34 BM candidates, we further verified several proteins in an independent set of samples by MRM.
Conclusion: We identified several BM candidates for COPD. Proteomic analyses of serum exosomes would provide novel strategy for biomarker discovery in the respiratory diseases.