ポスター発表
- 第1日 5月15日（火） ポスター会場
-
1P-01 PDF
化学修飾トリプシン由来想定外断片スペクトルライブラリーを利用した誤同定防止法
Chemically modified trypsin by reductive methylation is a standard reagent in protein identification by mass spectrometry. The reductive methylation of trypsin is supposed to protect the enzyme against autolysis. However, it has been reported that peptides with mono- or di-methylated Lys, or Asn residue at their C-termini were observed in autolysis products of chemically modified trypsin. In most cases, the chemical modification of trypsin is not considered in database searches. Modified trypsin-derived spectral data might be incorrectly assigned to peptides of other proteins, making false-positive identification. In this paper, we will present a novel and simple database search strategy to prevent false-positive identification by using a spectral library of unexpected peptides from chemically modified trypsin.