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第1日　5月15日（火）　17:20～17:40　A会場（オービットホール）

1A-O2-M-1720　　PDF

メタボロミクスデータの統合に向けた代謝物相対定量値の実験室間比較

(¹九大生医研・ ²阪大院情報・ ³慶大先端生命研・ ⁴理研・ ⁵東大院医・ ⁶エーザイ)
和泉自泰¹・ 松田史生²・ 平山明由³・ 池田和貴⁴・ 北芳博⁵・ 堀江勘太⁶・ 馬場健史¹・ ^o小田吉哉^5,6

One of the current problems in metabolomics is the difficulty in integrating data collected on different days, using different equipment and/or facilities. Accordingly, the development of a fundamental methodology that enables quantitative comparison of large scale data is needed for next-generation metabolomics study. As a starting point, we examined whether different analytical methods among different laboratories provide within tolerant relative quantification data for certain metabolites. Identical samples extracted from two human cancer cell lines (HeLa and MCF-7) were distributed to each facility, and hydrophilic and hydrophobic metabolites analyses were performed. Based on the preliminary results, we investigated the number of identified metabolites from cancer cells and the variations of relative quantification results (MCF-7/HeLa) among analytical methods.