オーラルセッション
- 第1日 5月15日（火） 16:20～16:40 A会場（オービットホール）
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1A-O2-M-1620 PDF
トランスオミクスを用いたインスリン作用機構の解明
Insulin action involves dynamic molecular interactions between multiple layers including protein phosphorylation, and metabolites. We performed metabolomic and phospho-proteomic analysis in insulin-stimulated Fao hepatoma cells, and automatically reconstructed global molecular network of insulin action by use of trans “omics” data together with several databases. We found a landscape of global network of insulin-dependent metabolic control that involves 13 protein kinases, 26 phosphorylated metabolic enzymes, and 35 allosteric effectors, resulting in quantitative changes in 44 metabolites (Yugi et al, Cell Rep. 2014, Yugi et al, Trends. Biotech., 2016). We also performed the transcriptomic analysis of insulin-dependent gene expression and found that selective regulation of up-regulated and down-regulated genes by temporal patterns and doses of insulin (Sano et al, Sci. Signal, 2016).