3P-25 PDF
Imaging Mass Spectrometry for the pathological studies of cardiac allografts
Rejection of transplanted heart tissue has always been the major obstacle to transplantation surgery. Once identified a type of heart rejection phenotype, it could lead to the development of new treatment strategies. To investigate distinct rejection patterns from transplanted cardiac tissue histology, biopsied samples in FFPE type specimen are the only resources to study pathology. Combined with pathological observations, here we challenged a comprehensive matrix-assisted laser desorption/ionization (MALDI) mass imaging mass spectrometry (IMS) approach at proteome level. This work explores the clinical feasibility of this technique and its relevance compared to existing clinical and pathological data sources. The main achievement must be a protocol generation for IMS targeting formalin-fixed, paraffin-embedded (FFPE) tissue samples.