3P-20 PDF
Development of novel MALDI matrix for in situ metabolomics
Recenly, matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) has been used not only for biopolymer analysis but also for low-molecular-weight metabolite analysis because it is a highly sensitive, high-throughput, and low sample-consuming technique. Mass spectrometry imaging (MSI) is one of the the most important application of MALDI, because this technique is now widely used for in situ imaging of endogenous or exogenous molecules such as proteins, lipids, drugs and their metabolites, and expected to be a potential tool for pathological analysis and understanding of disease mechanisms. In MALDI-MS analysis, the molecular coverage of detectable compounds is strongly associated with the molecular species of the matrix. At present, little is known about matrix capable of ionizing all compounds, and the detectable coverage is very narrow. Therefore, application of MALDI-MS for low-molecular-weight compound analysis is still limited to not many interesting compounds but analyzable ones using commercially available matrices.
Herein, we report the properties of novel synthesized matrices and ionization efficiency of representative low-molecular-weight metabolites such as amino acids and nucleotides in negative ionization mode MALDI-MS analysis.