3C-O2-1520（3P-41） PDF
Development of the Procedure of MALDI Mass Spectrometry Using Zeolite Matrix for Simultaneous Imaging of Multiple Anticancer Drugs
In order to evaluate the effectiveness of photodynamic therapy for anticancer drug resistant cancer cells, it is necessary to assess the accumulation of photosensitizer in cells. In this study, imaging mass spectrometry (IMS) using matrix-assisted laser desorption/ionization which can measure multiple drugs simultaneously was employed. For the ionization of anticancer drug, docetaxel, which has low ionization efficiency, the mixture of 6-aza-2-thiothymine (ATT) and NaY5.6 zeolite was used as the zeolite matrix (ATT/NaY). Moreover, in order to complete cellular scale IMS, spray method which can prepare the fine co-crystals between drugs and matrix was employed. At first, the simultaneous detection of docetaxel and photosensitizer, protoporphyrin IX (PpIX) from the mixture was examined. By using ATT/NaY as a matrix, the detection sensitivity of docetaxel was improved as compared with using only ATT, and the simultaneous detection of docetaxel and PpIX was succeeded. From this result, the effectiveness of ATT/NaY for the simultaneous detection was indicated. At the next step, the simultaneous detection of drugs administered in cancer cells was examined, but only PpIX was detected and docetaxel was not. For the simultaneous detection, the examination of the method of preparing cell suspension and matrix spraying will be performed.