3C-O1-1030（3P-39） PDF
Investigations of novel amino acids fragment ions using GC-MS/MS for precise 13C-metabolic flux analysis
Novel fragmentations of tert-butyldimethylsilyl (TBDMS)-amino acids were investigated by gas chromatography-tandem mass spectrometry (GC-MS/MS). The product ion spectra of 15 TBDMS-amino acids revealed 24 novel fragment ions. The amino acid-derived carbons included in the five fragment ions were identified by the analyses of 13C-labeled authentic standards. It was also confirmed that the precision of 13C-metabolic flux analysis in Escherichia coli central carbon metabolism could be improved by introducing 13C-labeling data derived from novel fragmentations.