3A-O1-0940（3P-31） PDF
Development of milieu-lipidomics platform for understanding mutual interaction between host and intestinal flora
Lipid metabolites consist of a wide variety of fatty acids or of polar groups, and some of them are quantitatively and qualitatively variable or oxidizable depending on intestinal bacteria. To investigate lipid metabolic changes in detail, lipidomics analysis by LC-MS has been wildly applied. However, it remains insufficient to measure them comprehensively and difficult to discover the important metabolic change unbiasedly by conventional approach. In this study, we designed a new lipidomics approach, which was named milieu-lipidomics, to grasp metabolic crosstalk between host and intestinal bacteria using LC/Q-TOF MS and Lipidiscovery (in-house screening software) with a high degree of the identification accuracy. Our strategy has a strong potential as an improved lipidomics strategy in order to understand mutual interaction between host and intestinal flora or to find important factors involved in the homeostasis.