2P-43 PDF
Application of Micofluidics/Tandem Quadrupole LC-MS/MS for MRM Based Translational Research Analysis of Putative Heart Failure Peptide Biomarkers in Human Plasma
The application of tandem quadrupole MS with microfluidic chromatography for the analysis of proteolytic peptides in human plasma is compared and contrasted with nanoscale LC and high-resolution oa-ToF MS configurations. A tandem quadrupole platform was considered for its performance in terms of sensitivity, selectivity, precision, and linearity. Microfluidic chromatography was selected as it afforded the optimum balance of sensitivity and throughput, whilst minimizing isobaric coelution, thereby providing an ideal LC-MS configuration for the application to large sample cohorts in translational studies. This LC-MS configuration was also utilized to demonstrate that proteolytically digested, non-depleted plasma samples from heart failure patients could be classified with good discriminative power using a subset of proteins previously suggested as candidate biomarkers for cardiovascular diseases.