2P-03 PDF
Identification of α,β-Unsaturated Carbonyl Compounds in Cigarette Smoke Using Michael Addition Reaction to Glutathione
Cigarette smoke contains many toxicants that cause oxidative damage in various cells and tissues. We have previously detected three α,β-unsaturated carbonyl compounds, methyl vinyl ketone (MVK), acrolein and crotonaldehyde, in aqueous nicotine/tar-free cigarette smoke extract (CSE). These compounds as well as CSE irreversibly modified glutathione (GSH), an important antioxidant, by the Michael addition reaction in mouse melanoma cells, causing a rapid depletion of intracellular GSH and leading to cell death.
In this study, we tried to presume the structure of other α,β-unsaturated carbonyl compounds contained in the CSE, by measuring the elemental composition of the Michael addition reaction products of CSE and GSH.