1A-W1-1830 PDF
Approaches to Reduce or Eliminate Matrix Effects in Quantitative LC/MS Analysis Focused on Instrumental Parameters, MS Ionization
High performance liquid chromatography (HPLC) coupled to mass spectrometry (MS) has become the predominant analytical method for the quantitative determination of analytes in biological matrices because of its high specificity, sensitivity, and throughput. However, matrix effects have become a major concern in quantitative liquid chromatography–mass spectrometry (LC/MS) because they detrimentally affect the accuracy, reproducibility, and sensitivity. Matrix effects occur when compounds that are coeluted with the analyte interfere with the ionization process in the MS detector, thereby causing ionization suppression or enhancement. Wherever possible, matrix effects should be reduced or eliminated by the optimization of chromatographic conditions, improving sample preparation and/or by changing the type of ionization employed.
In this workshop, we introduce the approaches to reduce or eliminate matrix effects in quantitative LC/MS analysis with some examples focused on instrumental parameters, MS ionization and ion source beyond the MS manufacturers.